Brentford striker Ivan Toney has received his first England call-up as Gareth Southgate announced his squad for the upcoming Nations League fixtures.
The Three Lions face Italy and Germany this month in preparation for the 2022 World Cup in November, adding extra significance to this squad.
Manchester United duo Jadon Sancho and Marcus Rashford were both left out, albeit for different reasons.
Both have returned to form at Old Trafford after losing their England spots last season and it is understood Southgate wanted to pick Rashford, but the forward did not pass a late fitness test.
Sancho, however, has been snubbed in favour of West Ham winger Jarrod Bowen, while Man City forward Jack Grealish also keeps his place.
Tottenham defender Eric Dier did earn a recall to the squad for the first time since 2020 after impressing under Antonio Conte.
However, there was no spot for Liverpool midfielder Harvey Elliott, who had been tipped for a senior bow.
Rashford is not the only player to miss out through injury as Reds captain Jordan Henderson and Everton goalkeeper Jordan Pickford are both sidelined.
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United defenders Harry Maguire and Luke Shaw keep their place after being dropped by Erik ten Hag, as does Chelsea left-back Ben Chilwell, while Liverpool right-back Trent Alexander-Arnold is included despite questionable form for his club.
With the 2022 World Cup in Qatar just nine weeks away, the autumn international break represents the last squad Southgate will name before the tournament.
The England boss selected 28 players and will narrow that down to 26 for the World Cup, leaving a number of players fighting for their seat on the plane.
Bees forward Toney became impossible to ignore after an electrifying start to the 2022/23 season and a number of injuries to English strikers.
The 26-year-old has five goals and two assists in six games, following on from an exceptional debut campaign in the top-flight.
Dean Henderson (Nottingham Forest)
Nick Pope (Newcastle)
Aaron Ramsdale (Arsenal)
Trent Alexander-Arnold (Liverpool)
Ben Chilwell (Chelsea)
Conor Coady (Everton)
Eric Dier (Tottenham)
Marc Guehi (Crystal Palace)
Reece James (Chelsea)
Harry Maguire (Man United)
Luke Shaw (Man United)
John Stones (Man City)
Fikayo Tomori (AC Milan)
Kieran Trippier (Newcastle)
Kyle Walker (Man City)
Jude Bellingham (Borussia Dortmund)
Mason Mount (Chelsea)
Kalvin Phillips (Man City)
Declan Rice (West Ham)
James Ward-Prowse (Southampton)
Tammy Abraham (Roma)
Jarrod Bowen (West Ham)
Phil Foden (Man City)
Jack Grealish (Man City)
Harry Kane (Tottenham)
Bukayo Saka (Arsenal)
Raheem Sterling (Chelsea)
Ivan Toney (Brentford)
With Callum Wilson, Dominic Calvert-Lewin, Patrick Bamford and Rashford all spending time on the sidelines this season, Toney emerged as the obvious choice.
Southgate will not take questions from the media on Thursday out of respect for the period of national mourning following the death of Her Majesty Queen Elizabeth II.